Cloning and functional research of renal cell carci - noma related novel gene - CYLZ-RCC18

摘要

After the renal cell carcinoma related novel gene fragment GYLZ-RCC18 was cloned by using suppres - sion subtractive hybridization (SSH), we used the SMART RACE technology to clone the full length of CYLZ-RCC18 And performed chromosome location by the FISH method. RT-PCR was used to detect the expression of the first read - Ing frame of GYLZ-RCC18 in different stages and grades of Renal cell carcinoma tissue and other tissues.