Retrovirus-mediated herpes simplex virus thymidine ki- nase gene therapy approach for hepatocellular carci- noma

摘要

The therapeutic effect of herpes simplex virus thymi- dine kinase/ganciclovir(HSV-tk/GCV)system on hepa- toecllular carcinoma was studied in this experiment. The tk-containing retroviral recombinants were used to infect hepatoma cells(BEL-7402)and the cells were treated with ganciclovir(0-1000μg/ml). The results showed that HSV- tk gene could be efficiently transferred in vitro into hep- atoma cells and stably expressed. The growth potential of the tk-containing cells was significantly inhibited by GCV(P < 0.01)as compared to the non-tk-containing cells.