Neurospora NADP-glutamate dehydrogenases and its ex - pressio in E. coli and trans - genic plants

摘要

Genes of NADP-glutamate dehydrogenase (NADP-GDH) were cloned from Neurospora intermedia (Ni), N. crassa (Nc), and N. sitophila (Ns). The sequences showed a High degree of homology at the cDNA and protein level. The Three GDH genes were cloned into pET30a and expressed in E. coli. The activity assay of purified GDH showed that the Ni-GDH hada higher activity and affinity to ammonia than Ns-GDH, and Nc-GDH.