High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensorst

摘要

We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage-DNA bioconjugates.DNA oligonudeotides were conjugated to M13 through acyl hydrazone linkages.In one case, DNAzymes retained their catalytic ability when anchored to the virus coat and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild conditions.