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Improvement of Chloroperoxidase Catalytic Activities by Chitosan and Thioglycolic Acid

机译：壳聚糖和巯基乙酸提高氯过氧化物酶的催化活性

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摘要

The chlorination and oxidation activity of chloroperoxidase (CPO) was enhanced 32 and 20% by chitosan, and oxidation activity 24% by thioglycolic acid, respectively. The pH optimum of chlorination reaction shifted 3 units to a less acidic environment by chitosan (from 2.75 to 5.75). The kinetic parameters indicated both the affinity and specificity of CPO to substrate were improved.
机译：壳聚糖将氯过氧化物酶（CPO）的氯化活性和氧化活性分别提高了32％和20％，硫代乙醇酸提高了24％。壳聚糖将氯化反应的最适pH值从3个单位移至酸性较低的环境中（从2.75至5.75）。动力学参数表明CPO对底物的亲和力和特异性均得到改善。

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  • 来源
    《Catalysis Letters》 |2009年第4期|457-461|共5页
  • 作者

    Chaohong Bai; Yucheng Jiang; Mancheng Hu; Shuni Li; Quanguo Zhai;

  • 作者单位

    School of Chemistry and Materials Science Shaanxi Normal University Chang’an South Road 199 710062 Xi’an Shaanxi People’s Republic of China;

    Key Laboratory of Macromolecular Science of Shaanxi Province Shaanxi Normal University Xi’an Shaanxi People’s Republic of China;

    Key Laboratory of Macromolecular Science of Shaanxi Province Shaanxi Normal University Xi’an Shaanxi People’s Republic of China;

    Key Laboratory of Macromolecular Science of Shaanxi Province Shaanxi Normal University Xi’an Shaanxi People’s Republic of China;

    Key Laboratory of Macromolecular Science of Shaanxi Province Shaanxi Normal University Xi’an Shaanxi People’s Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Chloroperoxidase; Activity; Chitosan; Thioglycolic acid; Kinetic parameters;

    机译：氯过氧化物酶;活性;壳聚糖;巯基乙酸;运动学参数;

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