Detection of k-ras gene point mutation in fine needle aspiration and pancreatic juice by sequence special primer method and its clinical significance

摘要

The point mutation rate of k-ras gene at codon 12 in pancreatic adenocarcinoma is reported to be as high as 90/100~[1-30], and with no mutations in normal pancreas tissues or other pancreatic disorders. We have detected the presence of k-ras gene mutation and its mutant styles since 1994 by PCR-SSP in the FNA or pancreatic juice obtained from pancreatic Adenocarcinoma tissues.