Screening of a Specific Monoclonal Antibody against and Detection of Listeria monocytogenes Whole Cells Using a Surface Plasmon Resonance Biosensor

摘要

In this study, a specific monoclonal antibody against Listeria monocytogenes was screened using an SPR biosensor. Monoclonal antibodies were bound to protein L, after which the L monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method, and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection of L monocytogenes. Under optimized conditions, 10~3 cells/mL or 50 cells were detected by the SPR biosensor.