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Attachment and response of human fibroblast and breast cancer cells to three dimensional silicon microstructures of different geometries

机译:人成纤维细胞和乳腺癌细胞对不同几何形状的三维硅微结构的附着和响应

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摘要

The paper reports the development of three dimensional (3-D) silicon microstructures and the utilization of these microenvironments for discriminating between normal fibroblast (HS68) and breast cancer cells (MDA-MB-231). These devices consist of arrays of microchambers connected with channels and were fabricated using a single-mask, single-isotropic-etch process. The behavior and response of normal fibroblast and breast cancer cells, two key cell types in human breast tumor microenvironments, were explored in terms of adhesion and growth in these artificial 3-D microenvironments having curved sidewalls. Breast cancer cells formed stable adhesions with the curved sidewalls however fibroblasts stretched and elongated their cytoskeleton and actin filaments inside the microchambers. Statistical analysis revealed that fibroblast cells grew on both flat silicon surfaces and inside the microchambers regardless rnof microchamber depth. However, the localization of breast cancer cells in these same substrates was dependent on the microchamber depth. After 72 h in culture, the ratio of the number of breast cancer cells on flat surfaces compared to breast cancer cells inside the microchambers was significantly decreased within the deeper microchambers; for microchambers having depths 88 μm less than 5% of the breast cancer cells grew on the flat surfaces. This behavior was sustained for 120 h, the longest time point examined. The results suggest that certain 3-D silicon microstructures have potential application as a tool to detect breast cancer cells and also as a platform for separating normal fibroblasts from breast cancer cells for cancer diagnosis applications.
机译:本文报道了三维(3-D)硅微结构的发展以及这些微环境在区分正常成纤维细胞(HS68)和乳腺癌细胞(MDA-MB-231)中的应用。这些设备由与通道连接的微腔阵列组成,并使用单掩模,单向同性蚀刻工艺制造。就人乳腺肿瘤微环境中的两种关键细胞类型,正常成纤维细胞和乳腺癌细胞的行为和反应,在这些具有弯曲侧壁的人工3-D微环境中的粘附和生长方面进行了研究。乳腺癌细胞与弯曲的侧壁形成稳定的粘附,但是成纤维细胞在微腔室内伸展并延长了其细胞骨架和肌动蛋白丝。统计分析表明,无论rnof微腔深度如何,成纤维细胞均在平坦的硅表面和微腔内部生长。但是,乳腺癌细胞在这些相同底物中的定位取决于微腔深度。培养72小时后,在较深的微腔室内,平坦表面上的乳腺癌细胞数量与微腔室内的乳腺癌细胞数量之比显着降低;对于深度为88μm的微腔室,小于5%的乳腺癌细胞生长在平坦表面上。这种行为持续了120小时,这是最长的时间点。结果表明,某些3-D硅微结构具有潜在用途,可作为检测乳腺癌细胞的工具,也可作为从乳腺癌细胞中分离正常成纤维细胞的平台,用于癌症诊断。

著录项

  • 来源
    《Biomedical Microdevices》 |2009年第2期|429-441|共13页
  • 作者单位

    Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA 24061, USA Virginia Tech MEMS Laboratory, The Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, VA 24061, USA;

    Biomedical Science, Edward Via Virginia College of Osteopathic Medicine, 2265 Kraft Drive, Blacksburg, VA 24060, USA;

    Virginia Tech MEMS Laboratory, The Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, VA 24061, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    MEMS; single mask; isotropic microchambers; cellular behavior; breast cancer;

    机译:MEMS;单面膜各向同性的微室细胞行为;乳腺癌;

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