Prophage lambda induction of Escherichia coli K12 envA uvrB: a highly sensitive test for potential carcinogens.

摘要

A simple, inexpensive, and sensitive test for potential carcinogens based upon the property of carcinogens to induce prophage lambda is described. By using chemicals activated with microsomal enzymes and E. coli K12 permeable (envA) tester bacteria also deficient in DNA repair (uvrB), the range of carcinogens detected in a lysogenic induction test (inductest) has been extended. We have provided the evidence that, after activation, carcinogenic polycyclic hydrocarbons such as benzo[a5pyrene and 7,12-dimethylbenz[a]anthracene induce prophage lambda. Three variants of the test have been developed (inductests I, II, and III), which are as sensitive as the mutagenicity test of Ames et al. [Ames, B. N., McCann, J. and Yamasaki, E. (1975) Mutat. Res. 31, 347-364]. Inductests II and III provide a quantitative estimation of the inducing activity of a carcinogen. With the latter test, one can determine: (i) the cellular toxic effect of a carcinogen and (ii) the kinetics of appearance and disappearance of active metabolites. For two series of chemicals, aflatoxins and benz[a]anthracenes, there is a good correlation between their carcinogenic activity in rodents and their prophage inducing activity in bacteria. The fact that the majority of the cell population is induced makes it possible to test the inducing activity of carcinogens at the biochemical level, e.g., by measuring lambda repressor inactivation.