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Mutant strains of Escherichia coli, a method of testing potential antibacterial agents using said strains as well as a testing kit

机译：大肠埃希氏菌的突变菌株，使用所述菌株检测潜在抗菌剂的方法以及检测试剂盒

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摘要

The subject of the present invention are mutant strains of Escherichia coli MG6165 (ATCC nr 47076) lacking all potassium transporters: Kdp, Kup and Trk (ΔKtrans), encompassing strains lacking at least one of the genes encoding mechanically gated channels mscS and mscL (ΔKtrans ΔmscS i/lub ΔmscL). In particular, it concerns Escherichia coli MG61655 (ATCC No. 47076) lacking all potassium transporters: Kdp, Kup and Trk (ΔKtrans), which additionally are devoid of the mechanically gated channel gene mscS—E. coli (ΔKtrans ΔmscS), the mechanically gated channel gene mscL—E. coli (ΔKtrans ΔmscL) and/or both the mechanically gated channel genes mscS and mscL—E. coli (ΔKtrans ΔmscS ΔmscL). The subject of the present invention is also a method of testing chemical compounds for potential antibacterial properties, making use of the abovementioned strains. Another subject of the present invention is a kit for testing chemical compounds as potential antibacterial substances, containing the abovementioned strains.

机译：本发明的主题是缺乏所有钾转运蛋白：Kdp，Kup和Trk（ΔKtrans）的大肠埃希氏大肠杆菌MG6165（ATCC nr 47076）的突变菌株，包括缺乏至少一种机械编码基因的菌株。门控通道mscS和mscL（ΔKtransΔmscSi / lubΔmscL）。特别是涉及缺乏所有钾转运蛋白：Kdp，Kup和Trk（ΔKtrans）的大肠杆菌 55616（ATCC编号47076），这些转运蛋白还缺少机械门控通道基因mscS- E．大肠埃希氏菌（ΔKtransΔmscS），即机械门控通道基因mscL- E。（ΔKtransΔmscL）和/或机械门控通道基因mscS和mscL-E。（ΔKtransΔmscSΔmscL）。本发明的主题还是利用上述菌株测试化合物潜在的抗菌性能的方法。本发明的另一个主题是一种试剂盒，用于测试含有上述菌株的化合物作为潜在的抗菌物质。 展开▼

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公开/公告号US9309575B2

专利类型

公开/公告日2016-04-12

原文格式PDF

申请/专利权人 PIOTR KOPROWSKI;ANDRZEJ KUBALSKI;WOJCIECH GRAJKOWSKI;
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申请/专利号US201113642121

发明设计人 PIOTR KOPROWSKI;WOJCIECH GRAJKOWSKI;ANDRZEJ KUBALSKI;
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申请日2011-04-23

分类号C12N1/21;C12Q1/18;C12R1/19;C07K14/245;C12N1/20;

国家 US

入库时间 2022-08-21 14:33:26

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