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Production and Characterization of Antifungal Compounds Produced by Lactobacillus plantarum IMAU10014

机译:植物乳杆菌IMAU10014产生的抗真菌化合物的制备与表征

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摘要

Lactobacillus plantarum IMAU10014 was isolated from koumiss that produces a broad spectrum of antifungal compounds, all of which were active against plant pathogenic fungi in an agar plate assay. Two major antifungal compounds were extracted from the cell-free supernatant broth of L. plantarum IMAU10014. 3-phenyllactic acid and Benzeneacetic acid, 2-propenyl ester were carried out by HPLC, LC-MS, GC-MS, NMR analysis. It is the first report that lactic acid bacteria produce antifungal Benzeneacetic acid, 2-propenyl ester. Of these, the antifungal products also have a broad spectrum of antifungal activity, namely against Botrytis cinerea, Glomerella cingulate, Phytophthora drechsleri Tucker, Penicillium citrinum, Penicillium digitatum and Fusarium oxysporum, which was identified by the overlay and well-diffusion assay. F. oxysporum, P. citrinum and P. drechsleri Tucker were the most sensitive among molds.
机译:从乳杆菌中分离出植物乳杆菌IMAU10014,该乳杆菌产生广谱的抗真菌化合物,在琼脂平板测定中所有这些化合物均对植物病原真菌具有活性。从植物乳杆菌IMAU10014的无细胞上清液中提取了两种主要的抗真菌化合物。通过HPLC,LC-MS,GC-MS,NMR分析进行3-苯基乳酸和苯乙酸2-丙烯基酯。乳酸菌产生抗真菌的苯乙酸,2-丙烯基酯是第一个报道。其中,抗真菌产品还具有广泛的抗真菌活性,即针对灰葡萄孢,克氏菌,德氏疫霉,塔克青霉,柠檬青霉,指状青霉和尖孢镰刀菌的抗真菌活性,这已通过覆盖和扩散良好的分析方法进行了鉴定。霉菌,柠檬青霉和德氏疟原虫Tucker在霉菌中最敏感。

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