A Schematic representation of lipid transport by the MlaABCDEF system in Gram-negative bacteria (LPS = Lipopolysaccharide). B 3.9 Å Cryo-EM map of MlaBDEFab-AppNHp in β-DDM. C Cartoon representation of the MlaBDEFab atomic model. The complex has a global C2 symmetry, with six copies of MlaD that span the inner membrane from the periplasmic space to the cytosol, two copies of MlaE embedded in the membrane (yellow/gold), two copies of the ATPase MlaF in the cytosol (green), each bound to a copy of MlaB (blue/cyan). D Top view of the MlaBDEFab complex reveals C6 symmetry of MlaD. E C-terminal regions of MlaF bind the opposing MlaB subunit via a handshake mechanism.
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