3D co-culture model of endothelial colony-forming cells (ECFCs) reverses late passage adipose-derived stem cell senescence for wound healing

摘要

3D culture of ASCs. a Schematic diagram of the experimental procedure for generating the 3D culture system in HA gel. Four percent HA gel was prepared and let dissolve for hours before ASC seeding. ASC suspension was seeded to make final HA concentration of 3%. After 24 h of culture, 3D structure could form in the HA gel. b Morphology of ASCs grown in monolayer culture at sequential passages (P0, 2, 4, 6, 8, 9). Scale = 100 μm