PSVI-23 Feeding enzymatically digested food waste altered fecal microbiota of growing-finishing pigs

摘要

Enzymatic digestion converted food waste from supermarkets into pasteurized liquid pig feed. This study was conducted to observe the fecal microbiota of growing-finishing pigs fed with enzymatically digested food waste. Fifty-six crossbred pigs (approximately 32.99 kg BW) were randomly assigned to one of the 2 dietary treatments with 7 replication pens and 4 pigs per pen. A 3-phase feeding program was used with d 0 to 28 as Phase 1, d 28 to 53 as Phase 2, and d 53 to 79 as Phase 3. The 2 dietary treatments were: a corn-soybean meal control diet, and a liquid diet produced from enzymatically digested food waste. Pigs were fed control or liquid diet in phases 1 and 2, while all pigs were fed with control diet in phase 3. Fecal samples were collected on d 0, 28, 53, and 79 from the same pig per pen and fecal microbiota was analyzed using 16S rRNA gene sequencing at the V4 hypervariable region and compositional data were analyzed using QIIME2 (2016.6). Observed species and Shannon diversity indexes were similar in both treatment groups on d 0, but pigs fed with food waste had greater (P < 0.05) Shannon diversity than control pigs on d 28, 53, and 79. Feeding food waste tended (P < 0.10) to decrease the relative abundance of Firmicutes compared with pigs fed control diet. Within this phylum, the abundance of Lachnospiraceae and Ruminococcaceae was increased (P < 0.05) and the abundance of Streptococcaceae and Clostridiaceae was decreased (P < 0.05). The analysis of Bray Curtis PCoA displayed a separate cluster between food waste and control groups on d 28 and 53. In summary, these results demonstrated that feeding enzymatic digested food waste modified the gut microbiome community of growing-finishing pigs.