For investigating the effect of Saccharomyces cerevisiae separated from massa medicata fermentata and sourdough, we studied the effects of newly developed composited yeast culture ( YC) , Bacillus subtilis ( BSK) and papain ( PE) on growth performance, nutrient apparent digestibility and fecal microflora number of nursery piglets. A total of 225 weaner piglets with initial body weight of (15.70±0.26) kg were randomly allotted to 5 groups with 3 replicates per group and 15 piglets per replicate. The control ( CT) group fed a basal diet, the YC groups fed the basal diet supplemented with 5 g/kg YC, the BSK group fed the basal diet supple⁃mented with 2×1011 CFU/kg BSK, the PE group fed the basal diet supplemented with 0.3 g/kg PE, and the complex ( YP) group fed the basal diet supplemented with 5 g/kg YC and 0.3 g/kg YP. The pretest period and test period lasted for 5 and 30 days, respectively. The results showed as follows:1) the final weight and aver⁃age daily gain ( ADG) of YP group were significantly higher than those of CT group ( P<0.05) , the feed to gain ratio (F/G) of YP group was significantly lower than that of CT group (P<0.05). The final weight of YC group was higher than that of CT, BSK and PE groups ( P>0.05) . There was no significant difference in average daily feed intake ( ADFI) among all groups ( P>0.05) . The diarrhea rate of YC, PE and YP groups was significantly lower than that of CT and BSK groups ( P<0.05) . 2) The apparent digestibilities of gross en⁃ergy ( GE) and dry matter ( DM) of YC, BSK, PE and YP groups were significantly higher than those of CT group (P<0.05), the apparent digestibilities of crude protein (CP) and phosphorus (P) of YC group were significantly lower than those of YP group (P<0.05), the apparent digestibilities of neutral detergent fiber ( NDF) and calcium ( Ca) of YC group were significantly lower than those of other groups ( P<0.05) . There were no significant difference in the apparent digestibilities of organic matter ( OM) and ether extract ( EE) a⁃mong all groups (P>0.05). 3) The fecal Escherichia coli number and Lactobacillus/Escherichia coli of YC and YP groups were significantly higher than those of other groups ( P<0.05) , and the fecal Escherichia coli number was significantly lower than that of other groups (P<0.05). In conclusion, the newly developed YC can improve the growth performance and intestinal micro⁃ecology environment of the piglets. The YC and PE combination can also improve the growth performance.%为了探究从神曲（ MMF）和酸面团中分离到的酿酒酵母的作用，本试验研究了新型的复合酵母培养物（ YC）、枯草芽孢杆菌（ BSK）、木瓜蛋白酶（ PE）对保育猪生长性能、营养物质表观消化率和粪便微生物数量的影响。选择平均体重为（15．70±0．26） kg的断奶仔猪225头，随机分为5组，每组3个重复，每个重复15头猪。对照（ CT）组饲喂基础饲粮，YC组饲喂基础饲粮＋5 g／kg YC，BSK组饲喂基础饲粮＋2×1011 CFU／kg BSK，PE组饲喂基础饲粮＋0．3 g／kg PE，复合（YP）组饲喂基础饲粮＋5 g／kg YC＋0．3 g／kg PE。预试期5 d，正试期30 d。结果表明：1） YP组末重、平均日增重显著高于CT组（ P＜0．05），料重显著低于CT组（ P＜0．05）；YC组末重高于CT、BSK、PE组（ P＞0．05）。各组间平均日采食量无显著差异（ P＞0．05）。 YC、PE和YP组的腹泻率均显著低于CT和BSK组（ P＜0．05）。2） YC、BSK、PE、YP组总能和干物质的表观消化率显著高于CT组（ P＜0．05），YC组粗蛋白质、磷的表观消化率显著低于YP组（ P＜0．05），YC组酸性洗涤纤维、钙的表观消化率显著低于其他各组（ P＜0．05）。各组间有机物和粗脂肪的表观消化率无显著差异（ P＞0．05）。3） YC、YP组粪便乳酸菌数量和乳酸菌／大肠杆菌显著高于其他各组（ P＜0．05），粪便大肠杆菌数量显著低于其他组（ P＜0．05）。由此可知，新型YC可以改善仔猪生长性能和肠道生态环境，YC和PE的联用对仔猪生长也有积极作用。
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