Clinically relevant dual probe difference specimen imaging (DDSI) protocol for freshly resected breast cancer specimen staining

摘要

Flow Cytometry Quantification of EGFR status in vitro & IHC validation of EGFR receptor expression ex vivo in the A431, AsPC-1 & 9L cell lines.a Flow cytometry-based analysis for the A431, AsPC-1 and 9L cell lines was completed for n = 3 samples per cell line to quantify EGFR receptor expression. b Serial sections of representative resected specimens from each xenograft type (A431, AsPC-1 and 9L) were stained with gold standard H&E and IHC to validate EGFR expression in tissue