METHOD FOR LOCATING INDIVIDUAL MOLECULES OF A DYE IN A SPECIMEN AND FOR GENERATING HIGH-RESOLUTION IMAGES OF A STRUCTURE IN A SPECIMEN

摘要

The present invention relates to a method for generating high-resolution images of a structure in a specimen or for locating individual molecules of a fluorescent dye in a specimen and to the use of a fluorescent dye in a method of this kind. The method according to the invention is distinguished in that the fluorescent dye is initially formed from a protected, non-fluorescent form of the dye prior to the scanning with excitation and fluorescence preventing light in a photoactivation reaction comprising at least two reaction steps and that the protected, non-fluorescent form of the dye is inert to the excitation and fluorescence preventing light.