ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy

摘要

DF-1 cells were infected with ALV-J for 24, 48, or 72 h and the expression levels of GADD45β were analyzed. Statistical differences were determined using two-way ANOVA.  **  ***  b Following 24, 48, or 72 h of ALV-J infection, total protein was extracted from DF-1 cells or CEF cells, the expression levels of GADD45β were analyzed using western blotting. The chart ( ) shows the quantification of GADD45β/β-actin in ( ). Cells were infected with ALV-J for 24 h and immunostained with rabbit anti-gp37 antibody (green). The expression levels of GADD45β (red) were analyzed using confocal microscopy. DAPI (blue) was used to stain the nuclear DNA. Scale bar, 10 μm. The chart ( ) shows the fluorescence intensity of endogenous GADD45β in ( ). All experiments were repeated in triplicate.