Overexpression of PGC-1α influences the mitochondrial unfolded protein response (mtUPR) induced by MPP+ in human SH-SY5Y neuroblastoma cells

摘要

The expression pattern (proteins and mRNA) of YME1L1, HSPA9, CLPP and HSPE1 in response to MPP  treatment. ( ) Relation of PGC-1α/LRPPRC pathway and the chaperones and proteases. ( – ) Changes in the chaperones and protease proteins were observed at various time points following cell treatment with 1000 µM MPP . ( ) The expression of YME1L1, HSPA9, CLPP and HSPE1 proteins at various time points was detected by immunoblotting, full-length blots/gels are presented in Supplementary Fig.  . ( ) The expression of YME1L1, HSPA9, CLPP and HSPE1 protein at various time points was detected by Western blotting. ( ) *P #P d) *P #P e) *P f) *P #P g,h,i) and ( ) Changes in mRNA levels of chaperones and proteases following treatment with MPP 1000 μM at each time point. ( ) *P ##P h) *P i) **P j) **P