Neuroprotective effects of schisanhenol against MPP~+-induced oxidative stress in human neuroblastoma SH-SY5Y cells

摘要

Oxidative stress plays an important role in Parkinson's disease (PD), suggesting that an antioxidant may be an ideal option for the treatment of PD. Schisanhenol (Sal), an active component from a Chinese herb, has significant antioxidant activity. In this study, we investigated the effect of Sal against oxidative stress induced by 1-methyl-4-phenylpyridinium ion (MPP~+) in human neuroblastoma SH-SY5Y cells. MPP~+-induced neuronal injury was characterized by the decrease of glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), increase of malondialdehyde (MDA) levels and the release of lactate dehydrogenase (LDH). Pretreatment with Sal markedly attenuated MPP~+-induced oxidative stress, indicated by the increase of GSH, GSH-Px, CAT, SOD activities, decrease of MDA levels and LDH release in the biochemical assay. Flow cytometry analysis demonstrated that Sal inhibited the production of reactive oxygen species (ROS) caused by MPP~+. The above effects were in a dose-dependent manner. These results suggest that Sal has neuroprotective effects against MPP~+-induced oxidative stress in SH-SY5Y cells, which might be a potential therapeutic agent for preventing or treating PD.