Methylenetetrahydrofolate Reductase CpG Islands: Epigenotyping

摘要

Determination of the differential DNA methylation patterns of methylenetetrahydrofolate reductase ( ) that are associated with differential MTHFR activity is important to understand the pathogenesis of ischemic stroke. However, to date, no data are available on the differential DNA methylation profiles of Kelantanese Malays. Therefore, we developed a rapid and efficient serial pyrosequencing assay to determine differential DNA methylation profiles of , which help to further our understanding of the pathogenesis of ischemic stroke. The developed assay also served as the validation platform for our previous computational epigenetic research on .