Distribution of Methylenetetrahydrofolate Reductase (MTHFR) C677T Polymorphism in Postmenopausal Indonesian Women with Osteoporosis - A Preliminary Study

摘要

Osteoporosis is a condition that is characterized by reduced bone mass and micro-architectural deterioration. Previous studies have shown that the MTHFR C677T polymorphism may be involved in the development of osteoporosis. The aim of this study was to characterise the distribution of this polymorphism in 100 Indonesian postmenopausal women. The MTHFR C677T polymorphism were determined in 25 women with osteopenia, 50 women with osteoporosis, and 25 control subjects using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism technique with Hinfl enzyme. The results showed CC genotype (wild type) 70%, CT genotype (heterozygous polymorphic) 29%, and TT genotype (homozygous polymorphic) 1%, with a T-allele frequency of 15.5%. The result also showed that there was no significant variation with MTHFR C677T genotype. It can be concluded that the MTHFR C677T polymorphism was weakly associated with BMD (Bone Mineral Density).