Integrated analysis of transcriptomic and metabolomic profiling reveal the p53 associated pathways underlying the response to ionizing radiation in HBE cells

摘要

Bioinformatic analysis of the mRNA expression alteration based on HBE cells with or without CRISPR/Cas9-mediated -knockout. p53 knockout efficiency was determined using western blotting. Quantitative measurement of differential expressed mRNA in HBE cells with (p53-/-) or without CRISPR/Cas9-mediated -knockout (p53-wt) after 4 Gy IR. . A heatmap was illustrated to show the differential expressed mRNA in HBE cells with (p53-/-) or without CRISPR/Cas9-mediated -knockout (p53-wt) after 4 Gy IR. Pink represents upregulated mRNA, and purple represents down-regulated mRNA. A volcano map was illustrated to show the differential expressed mRNA in HBE cells with (p53-/-) or without CRISPR/Cas9-mediated -knockout (p53-wt) after 4 Gy IR. Red represents upregulated mRNA, green represents down-regulated mRNA and grey represents the mRNA without expression change