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Effects of Wheat Bug (Eurygaster spp. and Aelia spp.) Infestation in Preharvest Period on Wheat Technological Quality and Gluten Composition

机译:收获前小麦Bug虫侵害对小麦技术品质和面筋成分的影响

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摘要

The effects of wheat bug infestation (Eurygaster spp. and Aelia spp.) on the composition of wheat gluten proteins and its influence on flour technological quality were investigated in the present study. Wheat samples of six wheat varieties, collected from two localities in northern Serbia, were characterized by significantly different level of wheat bug infestation. Composition of wheat gluten proteins was determined using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE), while the selected parameters of technological quality were determined according to standard and modified empirical rheological methods (Farinograph, Extensograph, Alveograph, and Gluten Index). The surface morphology of the selected samples was viewed using scanning electron microscopy (SEM). Wheat from wheat bug-infested locality regardless of the variety had deteriorated technological quality expressed with higher Farinograph softening degree, lower or immeasurable Extensograph energy, and Alveograph deformation energy. The most important changes in the gluten proteins composition of bug-infested wheat were related to gliadin subunits with molecular weights below 75 kDa, which consequently caused deterioration of uniaxial and biaxial extensibility and dough softening during mixing.
机译:本研究研究了小麦虫害(Eurygaster spp。和Aelia spp。)对小麦面筋蛋白组成的影响及其对面粉加工质量的影响。从塞尔维亚北部两个地方收集的六个小麦品种的小麦样品的特征在于,小麦虫害侵染程度明显不同。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS PAGE)确定小麦面筋蛋白的组成,同时根据标准和改进的经验流变方法(传真机,引伸计,酒精计和面筋指数)确定所选的技术质量参数。使用扫描电子显微镜(SEM)观察所选样品的表面形态。不论虫草品种如何,来自受虫害侵扰的地方的小麦,其粉质仪软化度较高,引伸仪能量较低或无法测量,并且测湿仪变形能较高,这表明技术质量下降。虫害小麦的面筋蛋白质组成中最重要的变化与分子量低于75 kDa的麦醇溶蛋白亚基有关,因此导致混合时单轴和双轴延伸性变差和面团软化。

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