Effects of pre-analytical heat treatment in factor VIII (FVIII) inhibitor assays on FVIII antibody levels

摘要

IntroductionThe use of pre-analytical heat treatment (PHT) with the Nijmegen-Bethesda assay (NBA) for inhibitors to factor VIII (FVIII) can remove/destroy infused or endogenous FVIII from patient plasma samples, allowing testing of recently infused patients with haemophilia. Two PHT methods have been described as follows: heating to 56°C for 30 minutes and heating to 58°C for 90 minutes. Data examining the effects of PHT on anti-FVIII IgG4, the antibodies known to correlate most closely with the presence of FVIII inhibitors, are limited.