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A general method for affinity purification of complement component C3b using factor H-sepharose.

机译：使用因子H-琼脂糖亲和纯化补体成分C3b的一般方法。

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摘要

Complement component C3b has been purified from human, rabbit and bovine serum by affinity chromatography on human factor H-Sepharose after preliminary fractionation by poly(ethylene glycol) and DEAE-Sepharose. The yields are high (35--40%) and the whole process is rapid (3 days). Binding of C3b to factor H-Sepharose is equimolar, has a sharp optimum pH at 7.6 and is quite sensitive to ionic strength.

机译：在通过聚乙二醇和DEAE-Sepharose初步分级分离后，通过人因子H-Sepharose的亲和色谱从人，兔和牛血清中纯化补体组分C3b。产率高（35--40％），整个过程快速（3天）。 C3b与因子H-Sepharose的结合是等摩尔的，在7.6时具有尖锐的最佳pH，并且对离子强度非常敏感。

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期刊名称 Biochemical Journal
作者
J D Scott; J E Fothergill;
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作者单位

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年(卷),期 1982(205),3
年度 1982
页码 575–580
总页数 6
原文格式 PDF
正文语种
中图分类分子生物学;
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专利

1. A general method for affinity purification of complement component C3b using factor H-sepharose [J] . J D Scott, J E Fothergill The biochemical journal . 1982,第3期

机译：使用H因子-琼脂糖亲和纯化补体成分C3b的通用方法
2. The mechanism of action of the C3b inactivator (conglutinogen- activating factor) on its naturally occurring substrate, the major fragment of the third component of complement (C3b) [J] . JD Gitlin, FS Rosen, PJ Lachmann The Journal of Experomental Medicine . 1975,第5期

机译：C3b灭活剂（凝集素原激活因子）对其天然底物（补体第三成分（C3b）的主要片段）的作用机理
3. The mechanism of action of the C3b inactivator (conglutinogen- activating factor) on its naturally occurring substrate, the major fragment of the third component of complement (C3b) [J] . JD Gitlin, FS Rosen, PJ Lachmann The Journal of Experomental Medicine . 1975,第5期

机译：C3b灭活剂（凝集素原激活因子）对其天然底物（补体第三成分（C3b）的主要片段）的作用机理
4. Complement factor H-related protein 2 (CFHR2) is a C3b and heparin binding protein [C] . Eberhardt H.U., Uzonyi B., Halbich S., European Congress of Immunology . 2009

机译：补体因子H相关蛋白2（CFHR2）是C3B和肝素结合蛋白
5. Generation of Multiple Fluid-Phase C3b:Plasma Protein Complexes During Complement Activation. Functional Significance and Possible Implications in C3 Glomerulopathies. [D] . Ramadass, Mahalakshmi. 2013

机译：补体激活过程中多个液相C3b：血浆蛋白复合物的产生。 C3肾小球病变的功能意义和可能意义。
6. The mechanism of action of the C3b inactivator (conglutinogen- activating factor) on its naturally occurring substrate the major fragment of the third component of complement (C3b) [O] . JD Gitlin, FS Rosen, PJ Lachmann 1975

机译：C3b灭活剂（凝集素原激活因子）对其天然底物（补体第三成分（C3b）的主要片段）的作用机理
7. A general method for affinity purification of complement component C3b using factor H-sepharose. [O] . Scott, J D, Fothergill, J E 1982

机译：使用因子H-琼脂糖亲和纯化补体成分C3b的一般方法。
8. Purification of Complement Components and Production of Anti-Complement Reagents [R] . 1967

机译：补体成分的纯化和抗补体试剂的生产

A general method for affinity purification of complement component C3b using factor H-sepharose.

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