Purification of Complement Components and Production of Anti-Complement Reagents

摘要

Since preliminary data indicated that complement (C') may play a role in tissue transplantation rejection, the following tasks were performed: (1) Studies were conducted, leading to the production of human complement (C') components for delivery to a selected group of investigators. Efforts were made to obtain a yield of 250 ml per month. If deemed feasible, pilot studies were to be made using guinea pig serum, since more detailed data were available in the literature regarding the isolation of these components. (2) Human complement components were produced which can most easily be separated and most readily be made available to investigators. (3) Techniques were developed for the production of all other components and pilot amounts of as many as may be feasible were produced. (4) Studies were conducted, leading to long-term preservation of complement components. (5) Quality-control parameters were defined for the production and storage of complement components.