首页> 美国卫生研究院文献>British Journal of Cancer >Prognostic significance of Ki-67 antigen and p53 protein expression in pancreatic duct carcinoma: a study of the monoclonal antibodies MIB-1 and DO-7 in formalin-fixed paraffin-embedded tumour material.
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Prognostic significance of Ki-67 antigen and p53 protein expression in pancreatic duct carcinoma: a study of the monoclonal antibodies MIB-1 and DO-7 in formalin-fixed paraffin-embedded tumour material.

机译:Ki-67抗原和p53蛋白在胰腺导管癌中的预后意义:福尔马林固定石蜡包埋的肿瘤材料中单克隆抗体MIB-1和DO-7的研究。

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摘要

Formalin-fixed paraffin-embedded material from 57 patients in whom curative resection of pancreatic carcinoma had been attempted was analysed by an immunohistochemical procedure to estimate proliferation and p53 protein expression. Using the monoclonal antibody (MAb) MIB-1, which recognizes a Ki-67 epitope, the proliferating cell index (PCI, percentage of immunoreactive tumour nuclei) and proliferating cell area (PCA, percentage of immunoreactive tumour nuclear area) were calculated using an interactive image analysis system and were compared with semiquantitative scoring of stainability. MAb DO-7, which recognizes both wild- and mutant-type p53 protein, was used to assess p53 expression in the same material. MIB-1 stainings were of high quality in 53 tumours. The median PCI was 29.7% (range 0.5-82.1%) and the median PCA was 10.6% (range 0.0-36.5%). There was a close correlation between PCI and PCA (P < 0.0001). PCI and PCA values were in conformity with the semiquantitative scoring (P < 0.0001). The p53 immunohistochemical stainings were successful in 48 tumours and the protein was expressed in 22 (46%). High PCI values (> 45%, n = 14) correlated with shorter survival time (P < 0.01). PCA (P < 0.05) and the expression of p53 protein (P < 0.001) were independent prognostic variables.
机译:用免疫组织化学方法分析了57例试图治愈胰腺癌的患者中福尔马林固定石蜡包埋的材料,以评估其增殖和p53蛋白表达。使用可识别Ki-67表位的单克隆抗体(MAb)MIB-1,使用ELISA方法计算增殖细胞指数(PCI,免疫反应性肿瘤细胞核的百分比)和增殖细胞面积(PCA,免疫反应性肿瘤细胞核的百分比)。交互式图像分析系统,并与染色的半定量评分进行了比较。识别野生型和突变型p53蛋白的单克隆抗体DO-7被用来评估p53在同一材料中的表达。 MIB-1染色在53个肿瘤中质量很高。 PCI的中位数为29.7%(范围为0.5-82.1%),PCA的中位数为10.6%(范围为0.0-36.5%)。 PCI和PCA之间存在密切相关性(P <0.0001)。 PCI和PCA值符合半定量评分(P <0.0001)。 p48免疫组织化学染色在48个肿瘤中成功,该蛋白在22个肿瘤中表达(46%)。高PCI值(> 45%,n = 14)与较短的生存时间相关(P <0.01)。 PCA(P <0.05)和p53蛋白的表达(P <0.001)是独立的预后变量。

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