A chemically synthesized gene for human interferon-gamma has been cloned into a prokaryotic expression vector under the regulation of a synthetic constitutive transcriptional-translational control unit that contains a strong bacteriophage T5 early promoter and a strong ribosome-binding site. Cells harboring the recombinant plasmid express high levels (4 X 10(9) units per liter of culture) of antiviral activity specific for interferon-gamma. Analysis of total cell lysates on NaDodSO4/polyacrylamide gels revealed a 17,200-dalton protein, expected for the nonglycosylated form of human interferon-gamma, that constitutes greater than 15% of total cell protein.
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机译：在合成的组成型转录-翻译控制单元的调控下，已将人干扰素-γ的化学合成基因克隆到原核表达载体中，该单元包含一个强噬菌体T5早期启动子和一个强核糖体结合位点。带有重组质粒的细胞表达高水平（每升培养物4 X 10（9）个单位）抗干扰素-γ特异性的抗病毒活性。在NaDodSO4 /聚丙烯酰胺凝胶上对总细胞裂解物的分析显示，人干扰素-γ的非糖基化形式有望达到17,200道尔顿的蛋白质，占细胞总蛋白质的15％以上。
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