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Monoclonal antibody against pertussis toxin: effect on toxin activity and pertussis infections.

机译:抗百日咳毒素的单克隆抗体:对毒素活性和百日咳感染的影响。

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摘要

Antibody-producing hybridomas of myeloma SP2/O and spleen cells of BALB/c mouse immunized with pertussis toxoid and pertussis toxin were selected by the binding ability of the monoclonal antibody to the subunit protein of the toxin. Two monoclonal antibodies, 1B7 and 3F10, specific for a subunit which has no binding activity to haptoglobin and sheep erythrocytes, named S1, and one antibody, 1H2, for a subunit related to the binding activity of the pertussis toxin molecule to haptoglobin or sheep erythrocytes, named S4, were examined for mouse protective activity against pertussis infection. Antibody 1B7 not only neutralized leukocytosis-promoting and islet-activating activities of the toxin but also protected mice against intracerebral and aerosol challenge with Bordetella pertussis. The antibody, furthermore, showed therapeutic effects on mice showing severe clinical signs with pertussis infection. The other two antibodies, 3F10 and 1H2, showed neither neutralizing nor protecting activity, nor significant synergistic effects on antibody 1B7.
机译:通过单克隆抗体与毒素亚基蛋白的结合能力,选择用百日咳类毒素和百日咳毒素免疫的骨髓瘤SP2 / O和BALB / c小鼠脾细胞的产生抗体的杂交瘤。有两种单克隆抗体1B7和3F10,一种对与触珠蛋白和绵羊红细胞没有结合活性的亚基具有特异性,一种名为1H2,针对与百日咳毒素分子与触珠蛋白或绵羊红细胞的结合活性有关的亚基检查了名为S4的小鼠对百日咳感染的保护作用。抗体1B7不仅中和了毒素的促进白细胞增多和胰岛激活的活性,而且还保护了小鼠免受百日咳博德特氏菌的脑内和气溶胶攻击。此外,该抗体对小鼠表现出治疗效果,该小鼠表现出严重的百日咳感染临床症状。其他两种抗体3F10和1H2既不显示中和活性,也不显示对抗体1B7的显着协同作用。

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