Twist和ZO-1在卵巢癌中的表达及其与 卵巢癌发生发展的相关性研究

摘要

目的：研究Twist和ZO-1在卵巢癌和正常卵巢组织中的表达及其分布特点，并探讨二者在卵巢癌表达中的相关性及其与肿瘤发生发展的关系。方法采用免疫组织化学方法（SP法）检测卵巢癌患者手术切除标本癌组织及正常卵巢组织中Twist和ZO-1的表达水平，并与肿瘤的分级和分期进行统计学分析。结果 Twist在卵巢癌组织中阳性表达率（76.0％，38/50）高于正常卵巢组织（20.0％，4/20），差异有统计学意义（P<0.05）；ZO-1在卵巢癌组织中阳性表达率（32％，16/50）低于正常组织（85.0％，17/20），差异有统计学意义（P<0.05）；Twist和ZO-1的表达水平在卵巢癌的不同分级和分期中的表达差异有统计学意义（P<0.05）；二者在卵巢癌中的表达呈负相关（r=-0.76， P<0.05）。结论 Twist和ZO-1在卵巢癌组织中表达的变化可能与肿瘤的发生发展有关；Twist和ZO-1联合检测可作为评估卵巢癌转移与预后的指标。%Objective To investigate the expression and distribution of Twist and ZO-1 in epithelial ovarian cancer and to explore the correlation between Twist and ZO-1 in ovarian cancer. Methods lmmunohistoehemical method(SP method)was used to detect the expression of Twist and ZO-1 in 50 cases of ovarian cancer tissue and 20 cases of normal tissue of patient.The clinicopathologic characteristics and tumor staging were statistically analyzed. Results The positive rate of Twist was76.0%(38/50)in ovarian cancer,20.0%(4/20)in the normal tissue of other patients,There were significant differences(P<0.05).The positive rate of ZO-1 was 32.0%(16/50)in ovarian cancer,significantly lower than that in the normal tissue 85.0%(17/20)(P<0.05).The expression of Twist and ZO-1 were associated with tumor staging and grading in ovarian cancer patients(P<0.05). There was a negative correlation between the expression of Twist and ZO-1 in ovarian cancer patients. Conclusions The the high express of Twist and low express of ZO-1 may be concerned with the genesis and the development of human epithelial ovarian cancer.The combined detection of Twist and ZO-1 expression may be used in assessing the metasasis and predicting the clinical prognosis of adenocarcinoma ovarian cancer.