The optimum conditions for extraction and separation of water-soluble nucleotide from fruit body of an edible fungus-Agrocybe cylindracea was studied. The crude nucleotides were assisted extraction with the grind bead . Three factors (grind time, ratio of liquid to solid, and loading capacity of grind beads) were investigated in the single-factor experiments. The process parameters were optimized by response surface analysis method. A multiple regression equation which was identified significant by regression analysis and the optimum extraction conditions were also determined (grind time: 12min, ratio of liquid to solid: 51:1 Ml/g, loading capacity of grind beads: 12.5%). The theoretical yield for nucleotide was 7.02%. Furthermore, the actual yield was 6.94%, which was in accordance with theoretical result and thereby illustrated the fitted multiple regression equation was credible. Compared with traditional methods, grind bead assisted extraction not only produced high yield of nucleotides, but also greatly shortened processing time, which provided an industrial reference for developing new processes of nucleotides production from edible fungi.%为了考察茶树菇中水溶性核苷酸的较优提取工艺,该文以食用真菌茶树菇子实体为原料,采用研磨珠细胞破壁提取法,对其所含的核苷酸类物质进行提取.以研磨时间、液料比及研磨珠装载量作为影响因素,采用响应面分析法进行条件优化,得到具显著性的拟合回归方程,并确定最佳提取工艺条件为:研磨时间12 min,液料比为51∶1 mL/g,研磨珠装载量为12.5％时,理论核苷酸得率为7.02％.并且,通过验证试验,实际得率为6.94％,结果与模型符合良好,进一步说明拟合方程的可靠.与传统方法相比,本文采用的研磨珠破壁提取方法,不仪所得核苷酸产品得率高,并且可大大缩短操作时间,为食用菌核苷酸的新型生产工艺的开发提供了理论参考.
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