Process optimization of automated large-scale oligonucleotide synthesis.

摘要

Oligonucleotides are a {dollar}4.5 billion segment of the biotech industry, used for a wide spectrum of medical applications and in drug development. Oligos will likely be the jumping off point for synthesizing DNA.; The objective of this first-ever study in the process optimization of polynucleic acids was to maximize the yield [and profit] by optimizing the pore diameter and the load density of the cpg solid phase support used to grow the oligos, for any given oligo length.; The optimal pore diameter lies at the greatest possible pore diameter and minimal possible load density, which are dependent on the oligo length.; This result was totally non-intuitive and contrary to the current operating point where a constant pore diameter and load density is used for all oligo lengths and sequences.