黔南烟草马铃薯Y病毒(PVY)株系的分子鉴定

摘要

为了揭示贵州省黔南烟区马铃薯Y病毒(PVY)株系分布及分子变异规律,先利用血清学方法初步鉴定PVY株系,再选择19个PVY分离物进行分子鉴定.结果表明,克隆的19个PVY cDNA片段全长均为1074 nts,包含1个801 nts的完整cp基因,编码266个氨基酸.序列比对发现,19个黔南PVY分离物cp基因核苷酸序列相似性为87.0％ ～ 99.9％,与GenBank登录的其他17个PVY株系cp基因序列相似性为86.6％ ～ 99.9％.系统进化树分析表明,黔南烟区19个PVY分离物中,有3个PVY分离物与PVYN株系和PVYNTN亲缘关系较近,而其他16个PVY分离物与PVYNN:O株系的亲缘关系最近.%To reveal the rules of distribution and molecular variation of strains of Potato virus Y (PVY) in Qiannan tobacco belt of Guizhou,PVY strains were initially identified by serological method; further,19 selected PVY isolates were subjected to molecular identification.The results showed that all the cloned fragments of the 19 PVY cDNA were 1074 nts in full-length,containing a complete cp gene of 801 nts and encoding 266 amino acids.The results of sequence comparison showed that the similarity of nucleotide sequence of cp genes in the 19 PVY isolates was 87.0％-99.9％,and the similarity with the cp genes in the 17 PVY strains logged in GenBank was 86.6％-99.9％.Phylogenetic tree analysis indicated that 3 of the 19 PVY isolates from Qiannan had closer relationship with PVYN and PVYNTN,and the other 16 PVY isolates were more closely related to PVYN N:O.