CRISP2基因在不同类型精子发生障碍中的表达及其意义

摘要

Objective To study sperm mRNA transcript expression of CRISP2 gene in the semen of patients with different types of spermatogenetic failure. Methods A total of 150 male infertility patients were divided into normal group, asthenozoospermia group and oligozoospermia group(n=50 for each group). Total RNA was extracted from sperm cells. Re-al-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) method was used to detect CRISP 2 gene mRNA levels in three groups. Results There was a significantly higher expression of CRISP2 in normal group (10.281 ± 2.173) than that of asthenozoospermia group (2.092±0.969, P<0.05). There was no significant difference in the expression of CRISP2 between normal group and oligozoospermia group (9.420±2.794, P>0.05). The relative expression of CRISP2 was sig-nificantly lower in asthenozoospermia group than that of oligozoospermia group (P<0.05). Conclusion The CRISP2 expres-sion was significantly different in patients with different types of spermatogenic failure. The reduced expression of CRISP 2 may lead to the decreased sperm motility.%目的：探讨不同类型精子发生障碍男性精液中CRISP2 mRNA的表达水平及其意义。方法150例男性不孕患者分为正常组、弱精子组、少精子组各50例，提取精液细胞总RNA，通过实时荧光定量聚合酶链式反应（qRT-PCR）测定3组精子中CRISP2基因的mRNA转录水平并进行比较。结果 CRISP2在正常组中的相对表达量（10.281±2.173）显著高于弱精组（2.092±0.969），差异有统计学意义（P<0.05），正常组与少精子组（9.420±2.794）差异无统计学意义（P>0.05），而弱精子组的CRISP2相对表达量也显著低于少精组（P<0.05）。结论 CRISP2在不同精子发生障碍男性中的表达显著不同，CRISP2表达的减少可能导致精子活力下降。