响应面法优化三叶青总黄酮的提取工艺研究

摘要

ABSTRACT:OBJECTIVE To optimize the extraction technics of total flavonoids from Radix Tetrastig-mae.METHODS Based on single factor experiments , extraction conditions includ liquid-solid ratio , ethanol con-centration ,extraction time and extraction temperature were optimized using response surface methodology.RESULTS The main factor of influencing total flavonoids extraction was liquid-solid ratio followed by extraction time.The op-timal extraction conditions were as follows:liquid-solid ratio 14.25 mL· g -1 ,ethanol concentration 50.7%,extraction time 1.975 h and extraction temperature 89.4℃.CONCLUSION Optimization of total flavonoids extraction from Radix Tetrastigmae by response surface methodology was steady ,reasonable and practicable.%目的：优选三叶青总黄酮的提取工艺。方法以液料比、乙醇浓度、提取时间、提取温度4个单因素为考察基础，利用响应面法优化并建立了三叶青总黄酮最佳的回流提取工艺。结果液料比对总黄酮提取量的影响最大，提取时间次之。最佳工艺条件为：液料比14.25mL· g －1，乙醇浓度：50.7％，提取时间：1.975h，提取温度：89.4℃。结论采用响应面分析法优选三叶青总黄酮的提取工艺稳定、合理、可行。