RP－HPLC法测定小儿清肺止咳片中栀子苷的含量

摘要

OBJECTIVE To establish the RP-HPLC method for determination of geniposide in Xiaoer Qingfei Zhike Pian.METHODS A RP-HPLC method was established.The chromatographic column was Diamonsil-C18 .The mobile phase was a mixture of acetonitrile–water(12∶88,V/V).The flow rate was 1.0mL・ min-1 ,and the detec-tion wavelength was 240 nm.RESULTS The linear range of geniposide was 5.0~50.0μg・ mL-1 ( r=0.9998 ) .The average recovery for geniposide was 100.4%, precision of the method was 0.44%( RSD, n =6 ) .CONCLUSION The method can be used for quantitative determination of the preparation.%目的：建立测定小儿清肺止咳片中栀子苷含量的方法。方法采用反相高效液相色谱法，Diamonsil－C18柱，乙腈－水（12∶88）为流动相，流速为1．0mL・ min －1，检测波长为240nm，柱温为30℃。结果栀子苷浓度在5．0～50．0μg・ mL －1间具有良好的线性关系（r＝0．9998），栀子苷平均回收率为100．4％，方法精密度（RSD）为0．44％（n＝6）。结论该法可用于小儿清肺止咳片中栀子苷的含量测定。