林下山参滴丸的含量测定及指纹图谱研究

摘要

测定林下山参滴丸的人参总皂苷及主要单体人参皂苷的含量，同时建立林下山参滴丸的指纹图谱。采用紫外－可见分光光度法测定林下山参滴丸中人参总皂苷的含量；采用高效液相色谱法（HPLC ）测定林下山参滴丸中3种主要单体人参皂苷的含量；采用HPLC法对10批次林下山参滴丸进行标准指纹图谱分析，通过中药色谱指纹图谱相似度评价软件（2004 A版）进行评价，并建立林下山参滴丸的共有指纹图谱。林下山参滴丸中人参总皂苷的含量为16mg／g ；人参皂苷Rg1、Re和Rb1的含量分别为2.667mg／g、0.433mg／g和5.100mg／g；指纹图谱有16个共有峰，稳定性、重复性、精密度考察中每个共有峰相对保留时间和相对峰面积的RSD均小于4％，10批次供试品相似度大于0.95。林下山参滴丸中富含人参总皂苷及单体人参皂苷营养成分；林下山参滴丸指纹图谱可有效控制其质量。%The aims of this paper are to determine the contents of ginsenosides and to establish the fingerprint of Linxiashanshen Pills .The deter-mination of total ginsenosides was determined by UV -Visible spectrophotometry .HPLC -PDA was chosen to detect the contents of gin-senosideRg1、Re and Rb1 .A total of10 batches of Linxiashanshen Pills were determinedby HPLC and were evaluatedby the Chromatographic Fin-gerprint Similarity Evaluation System (2004A Edition ) .As a result ,the standard fingerprint were established .The contents of total ginsenosies were determined as 16mg/g .The contents of ginsenosides Rg1 ,Re and Rb1 were respectively 2.667mg/g ,0.433mg/g and 5.100mg/g .The standard fingerprint including 16 characteristic peaks was established .RSD of relative retention time and relative peak areas in the precision ,re-producibility and stability test were less than 4% .The similarity result of analysis is more than 0.95 .This method can be used for the content determination of ginsenosides of Linxiashanshen Pills .In addition ,suitable standard fingerprint was obtained which can be used for the quality control of Linxiashanshen Pills .