Objective To investigate the effect of music electroacupuncture on perifocal ferric ions and neuronal apoptosis and the brain water content in rats with acute cerebral hemorrhage and explore the mechanism of its action on acute cerebral hemorrhage.Methods One hundred and twenty-eight healthy adult male Wister rats were randomized to normal, model, electroacupuncture and music electroacupuncture groups. In each group, four time points of 6 hrs, 24 hrs, 3 days and 7 days were set up, eight rats each time point. A rat model of acute cerebral hemorrhage was made using collagenase. In the electroacupuncture and music electroacupuncture groups, point Baihui was connected to the anode and point Taiyang to the cathode, 2 voltage was used as a selection parameter and electroacupuncture lasted 30 min. Intracerebral perifocal ferric ions and neuronal apoptosis and the brain water content were measured at four time points of 6 hrs, 24 hrs, 3 days and 7 days after model making.Results Intracerebral ferric ion content was higher in the model, electroacupuncture and music electroacupuncture groups of rats than in the normal group at 6 hrsafter model making (P<0.05), lower in the electroacupuncture and music electroacupuncture groups than in the model group at 24 hrs, 3 and 7 days after model making (P<0.05) and lower in the music electroacupuncture group than in the electroacupuncture group at 3 and 7 days after model making (P<0.05). Intracerebral neuronal apoptosis was higher in the model, electroacupuncture and music electroacupuncture groups of rats than in the normal group at 6 hrs after model making (P<0.05) and lower in the music electroacupuncture group than in the electro- acupuncture group at 7 days after model making (P<0.05). The brain water content was higher in the model, electro- cupuncture and music electroacupuncture groups of rats than in the normal group at 6 and 24 hrs after model making (P<0.05), lower in the electroacupuncture and music electroacupuncture groups than in the model group at 3 days after model making (P<0.05) and lower in the music electroacupuncture group than in the electroacupuncture group at 7 days after model making (P<0.05).Conclusions Music electroacupuncture has a benign regulating effect on intracerebral perifocal ferric ions, inhibits neuronal apoptosis around hematoma in the early stage and reduces cerebral edema in rats with acute cerebral hemorrhage. Its therapeutic effect is superior to that of electroacupuncture.%目的 观察音乐电针对急性脑出血大鼠脑内灶周铁离子变化、神经元细胞凋亡和脑组织含水量变化的影响,探讨音乐电针对急性脑出血的作用机制.方法 将128只健康成年雄性Wistar大鼠随机分为正常组、模型组、电针组和音乐电针组,每组分为6 h、24 h、3 d、7 d 4个时间点,每个时间点8只.应用胶原酶法制备急性脑出血大鼠模型.电针组和音乐电针组正极接百会穴,负极接太阳穴,选择参数为2 V,通电时间为30 min.于造模后6 h、24 h、3 d、7 d 4个时间点观测脑内灶周铁离子变化的水平、灶周神经元细胞凋亡及脑组织含水量的变化.结果 造模后6 h、24 h、3 d、7 d,模型组、电针组、音乐电针组大鼠脑内铁离子含量、神经元细胞凋亡均高于正常组(P<0.05),24 h、3 d、7 d时电针组、音乐电针组均低于模型组(P<0.05),3 d、7 d时音乐电针组低于电针组(P<0.05).造模后6 h、24 h、3 d、7 d,模型组、电针组、音乐电针组大鼠脑组织含水量均高于正常组(P<0.05),3 d时电针组、音乐电针组均低于模型组(P<0.05),7 d时音乐电针组低于电针组(P<0.05).结论 音乐电针对急性脑出血大鼠脑内灶周铁离子有良性调节作用,抑制脑出血后早期血肿周围部位神经元细胞凋亡的发生,减轻脑水肿,疗效优于电针组.
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