荧光定量PCR检测非小细胞肺癌中MTA1和E-cadherin的表达及意义

摘要

目的 探讨肿瘤转移相关蛋白家族1(MTA1)和E-钙黏蛋白(E-cadherin)在人非小细胞肺癌(NSCLC)中的表达及意义.方法 采用实时荧光定量PCR(Q-PCR)联合检测40例NSCLC组织、12例癌旁组织、12例良性病变肺组织中MTA1和E-cadherin mRNA表达的相对水平,分析他们的表达与临床组织病理学特征的关系.结果 MTA1 mRNA在NSCLC中平均表达水平高于癌旁和良性疾病组织(P＜0.05)；E-cadherin mRNA在NSCLC中平均表达水平低于癌旁和良性疾病组织(P＜0.05)；在NSCLC中MTA1和E-cadhefin的表达呈负相关(r=-0.561,P＜0.05)；在癌旁组织和良性疾病组织中MTA1和E-cadhefin的表达无统计学差异(P＞0.05)；MTA1和E-cadhefin mRNA表达水平与淋巴结转移及临床分期有关(P＜0.05).结论 MTA1过度表达及E-cadhefin表达的下调或缺失与NSCLC浸润和转移呈正相关,联合检测NSCLC中MTA1和E-cadhefin的表达,可用于预后评估.%Objective To explore the expression of MTA1 and E-cadherin genes and their significance in human non-small cell lung cancer (NSCLC). Methods Employ the real-time fluorescence quantitative polymerase chain reaction (Q-PCR) to examine the expression of MTA1 and E-cadherin mRNA in 40 samples of NSCLC tissues, 12 samples of paracarci-noma tissues and 12 samples of benign lung tissues, and to evaluate the relativity of MTA1 , E-cadherin expression and NSCLC clinical pathologic features. Results The average expression level of MTA1 mRNA in NSCLC was higher than that in the paracarcinoma tissues and the benign lesions tissue (P < 0. 05). The average expression level of E-cadherin in NSCLC was lower than that in the paracarcinoma tissue and the benign lesions tissue (P < 0. 05). In NSCLC tissues the expression of MTA1 and E-cadherin was negatively correlated (r=-0.561, P < 0. 05). There was no statistical difference in the MTA1 and E-cadherin expression between the cancer and benign disease tissue. The expression of MTA1 and E-cadherin mRNA was closely relevant to lymph node metastasis and the clinical stages (P <0. 05). Conclusions The overex-pression of the MTA1 gene and the loss or lack expression of the E-cadherin were correlated with invasion and metastasis of NSCLC. Both of the expression of MTA1 and E-cadherin could work together for prognosis assessment.