靶向HER2基因的siRNA联合卡铂对肺腺癌A549细胞的抑制作用

摘要

目的 探讨靶向HER2基因的siRNA联合卡铂对肺腺癌A549细胞的抑制作用.方法 设计并合成3对靶向HER2基因的siRNA(2621组、1724组、1491组),分别转染肺腺癌A549细胞,同时设阴性对照组及空白组.应用实时荧光定量PCR法和免疫组化法检测细胞内HER2 mRNA及其蛋白的表达.应用流式细胞术检测卡铂与HER2 siRNA联合应用时肺癌A549细胞的凋亡率.结果 2621组和1724组HER2 mRNA及蛋白表达均低于1491组和阴性对照组.流式细胞术检查显示,各组间细胞凋亡率比较P＜0.01,HER2 siRNA+卡铂组细胞的凋亡率均高于对应浓度的卡铂组.结论 siRNA能抑制HER2基因在肺癌A549细胞中的表达,并与卡铂协同诱导癌细胞凋亡.%Objective To investigate the inhibition of human lung adenocarcinoma cell line A549 by siRNA targeting for HER2 gene combining with carboplatin (CBP) and to provide experimental basis for the clinical treatment of lung cancer patients. Methods 3 pairs of HER2 siRNA(2621 group, 1724 group and 1491 group) were designed and composed. The siRNA was transfected into A549 cells, and the mRNA and protein levels of HER2 were detected by real-time RT-PCR and flow cytometry, respectively. Dealt with CBP and HER2 siRNA, the rate of apoptosis was tested by flow cytometry. Restuls The mRNA and protein levels of HER2 in 2621 group and 1724 group were lower than those in other groups. The rate of apoptosis in HER2 siRNA + CBP group was higher than that in other groups with CBP in corresponding concentration. Conclusions SiRNA targeting HER2 gene can effectively inhibit HER2 expression in A549 cells, and can induce the apoptosis of the cells combining with CBP.