五味子乙素对脑胶质瘤细胞U87增殖的影响及其机制探讨

摘要

目的：观察五味子乙素( Sch B)对脑胶质瘤细胞U87增殖的影响,并探讨其可能机制。方法培养U87细胞,分为A、B、C、D组,D组加入含5%血清的培养液200μL,A、B、C组分别加入含50、100、200μg/mL Sch B的等量培养液,比较各组细胞增殖情况、细胞周期及细胞中Caspase-3、Bax、Bcl-2及Bax/Bcl-2。结果与D组比较, A、B、C组48 h和72 h的细胞OD值降低(P均<0.05)。与A、D组比较,B组Caspase-3、Bax、Bax/Bcl-2的A值及阳性表达率高(P均<0.05)。与D组比较,C组Caspase-3、Bax/Bcl-2的A值及阳性表达率高(P均<0.05)。与A组相比,C组Caspase-3的A值及阳性表达率高(P均<0.05)。与D组比较,A组G1期、G2/M期细胞比例增加,P均<0.05。结论 Sch B能明显抑制U87细胞增殖,诱导其凋亡,且呈一定的量效关系；其机制可能与上调细胞Caspase-3、Bax、Bcl-2表达有关。%Objective To investigate the effect of Schisandrin B ( Sch B) on the proliferation of glioma U87 cells and to explore its possible mechanism. Methods Glioma U87 cells were cultured and were divided into groups A, B, C and D. Group D was added with culture solution that containing 5% serum 200 μL, and groups A, B and C were added with the same amount of nutrient solution which respectively contained 50, 100 and 200μg/ml Sch B. The cell proliferation was compared between these groups. The cell cycle and the expression of Caspase-3, Bax, Bcl-2 and Bax/Bcl-2 were respec-tively detected by ELISA and immunohistochemistry. Results Compared with group D, the A value at 48 h and 72 h in the groups A, B and C was decreased. Compared with groups D and A, the A values of Caspase-3, Bax and Bax/Bcl-2 and the positive expression rate of group B were increased (all P<0. 05). Compared with group D, the A values of Caspase-3 and Bax/Bcl-2, and the positive expression of group C were increased (all P<0. 05). Compared with group A, the A val-ue of Caspase-3 and the positive expression rate of group C were increased (all P<0. 05). Compared with group D, the cell percentage of the G1 and G2/M phases of group A was increased, all P<0. 05. Conclusion Sch B can significantly inhibit the proliferation of U87 cells, induce the apoptosis, and it has a dose-effect relationship, whose mechanism may be related with the up-regulation of Caspase-3, Bax and Bcl-2 expression.