洋葱细胞质雄性不育基因分子标记的开发

摘要

The specific primers of orfA501 in chives and cytoplasmic male sterile line of onion were used to PCR amplification.The amplified fragment was extracted and sequenced.The primers were designed ac-cording to the obtained sequence for genome walking,and partial flanking sequence was obtained.Then a SCAR marker was developed and named as OC2175.With this marker,a special fragment of 2 175 bp could be obtained from the male sterile lines and a 1 053 bp fragment was amplified from both (s)-cytoplasm and (N)-cytoplasm.Seventeen pairs of male sterile lines and their corresponding maintainer lines with different genetic backgrounds were used to verify this SCAR marker.The results indicated that the SCAR marker was perfectly suitable for distinguishing the cytoplasm types S or N in onion.%根据细香葱 orfA501开发的特异引物，以洋葱细胞质雄性不育系为试材进行 PCR 扩增，对获得的片段进行回收测序，根据测序结果设计引物进行染色体步移，获得部分侧翼序列，开发了鉴定洋葱细胞质基因型的 SCAR 标记，命名为 OC2175。该标记在洋葱不育系中扩增出一条2175 bp 的特异条带和1053 bp 的条带，而保持系中仅扩增出1053 bp 的条带。对17组不同遗传背景的不育系及其相应的保持系进行验证，该SCAR 标记的鉴定结果与田间表型判断结果完全吻合。