贵州糯质小麦的分子标记辅助育种研究

摘要

糯性小麦具有优异的酿酒特性.目前贵州省内缺乏具有推广价值的糯性小麦,利用分子标记辅助育种,可有效提高选育适合贵州生态气候的糯性小麦新材料的效率.本研究利用与小麦糯质基因(Wx-A1a,Wx-B1a和Wx-D1a)连锁的特异分子标记,对俞糯麦×0117的F2代群体进行Wx基因位点扫描鉴定.结果发现:在显著水平p＞0.05时,3个糯质基因位点在F2代中的分离比例符合预期比例.在56个F2代分离群体中,3个基因位点都存在的材料有4株(7.14％);单缺失Wx-A1a或Wx-B1a或Wx-D1a基因位点的材料编号分别为5(8.93％)、8(14.29％)和19(33.93％);Wx-A1a和Wx-B 1a基因位点同时缺失的材料为5株(8.93％);Wx-A1a和Wx-D1a基因位点同时缺失材料为4株(7.14％);Wx-B1a和Wx-D 1a基因位点同时缺失材料为11株(19.64％);3个基因住点都缺失的全糯质单株未发现.%Waxy wheat has excellent characteristics in brewing,but greatly lacked applications in agricultural industry.It is great significance that breeding new varieties (lines) with good comprehensive traits to suit Guizhou ecological climate.In this study,Wx gene locus was used to identify F2 populations of Yuyou wheat×0117 using the specific molecular markers linked to wheat waxy genes (Wx-A1a,Wx-B1a and Wx-D1a).The results showed that the ratio of the three waxy genes in the F2 generation was in accordance with the expected ratio at the significant level of p＞0.05.4 plants have all of the three Wx genes,the number of plants lacked of Wx-A1a,Wx-B1a or Wx-D1a were 5,8 and 19 respectively;5 plants lacked both Wx-A1a and Wx-B1a;4 plants lacked both Wx-A1a and Wx-D1a;11 plants lacked bothWx-B1a and Wx-D1a;No plants lacked these three Wx genes.