飞龙掌血多糖清除自由基活性的研究

摘要

目的：研究飞龙掌血多糖的抗氧化活性。方法：采用Fenton法、DPPH法测定飞龙掌血多糖清除羟基自由基（.OH）、二苯代苦味酰肼自由基（DPPH.）的能力,以VC为对照。结果：质量浓度在0.2～0.4mg/mL范围内,飞龙掌血多糖随浓度增大,清除.OH能力增强,且0.4mg/mL时清除率高达73.7%;质量浓度在5×10-3～5×10-1mg/mL时,对DPPH.的清除率为13.5%～79.5%,清除作用与浓度呈正相关,当浓度为0.5mg/mL时,清除率高达79.5%。结论：飞龙掌血多糖具有较强的体外抗氧化性能,对.OH、DPPH.具有明显的清除作用。%Objective：To study the antioxidant activity of polysaccharides from Toddalia asiatica.Methods：The research adopted Fenton method and DPPH method to determine the scavenging ability of Toddalia asiatica polysaccharides to ·OH and DPPH· by comparing with VC.Results：In the concentration of 0.2 ~ 0.4mg/mL,the scavenging ability to ·OH was a positive correlation with the concentration of Toddalia asiatica polysaccharides,when the concentration was 0.4mg/mL,the clearance rate of ·OH was 73.7%.In the concentration of 5×10-3 ~ 5×10-1mg/mL,the clearance rate of DPPH· was 13.5% ~ 79.5%,the scavenging ability to DPPH· was also positively correlated with the concentration of Toddalia asiatica polysaccharides,when the concentration was 0.5mg/mL,the clearance rate of DPPH· was 79.5%.Conclusions：Toddalia asiatica polysaccharides had strong antioxidant properties and possessed a clear scavenging effect to ·OH and DPPH·.