高效液相色谱法测定益之康胶囊中总蒽醌(大黄素和大黄素甲醚)的含量

摘要

建立益之康胶囊中总蒽醌含量测定的高效液相色谱法.采用HPLC测定益之康胶囊中2种蒽醌类成分大黄素和大黄素甲醚的含量;流动相为甲醇-0.1％磷酸溶液(75∶25,v/v),柱温30℃,流速1.0 mL/min,检测波长254 nm.大黄素和大黄素甲醚分别在0.054～0.65 μg和0.022～0.27 μg范围内呈良好的线性关系,相关系数分别为0.9999和0.9999,检出限分别为0.86 μg/g和0.87μg/g,平均回收率分别为97.20％(RSD=2.26％)和99.96％(RSD=2.18％).结论:该方法简便、准确、可靠.%Objective:To establish a High Performance Liquid Chromatography (HPLC)method for determining the totalanthraquinone in Yizhikang capsule.Methods:The contents of emodin and physcion were determined by a HPLC method.The emodin and physcion were separated on a Waters Sunfire C18 column(250 mm ×4.6 mm,5 μm)using a mobile phase made up of methanol and 0.1％ phosphoric acid solution (75∶ 25,v/v)at a flow rate of 1.0 mL/min and detected by a UV detector at a wavelength of 254 nm.The column temperature was set at 30 ℃.Results:The response showed good linear relationship in the range of 54.09～649.05 μg/mL for emodin,22.11 ～265.31 μg/mL for physcion with all the coefficients of correlation (R2) =0.9999.The detection limits(LODs)were 0.86 μg/g for emodin,0.87 μg/g for physcion,respectively.The average recoveries of emodin and physcion were 97.20％ (RSD =2.26％) and 99.96％ (RSD =2.18％),respectively.Conclusions:This method is simple,accurate,reliable and reproducible and can be used for the quality control of yizhikang capsule.