响应曲面法对产蓝色素链霉菌YLA0的高产色素条件优化

摘要

Streptomyces sp.YLA0 was used as the experimental material to optimize the culture condition of high yield blue pigment.Based on the single factor experiment,C/N ratio,pH and culture time as three single factors were selected and optimized by Box-Behnken response surface design.The results showed that the optimal culture conditions of Streptomycin sp.YLA0 were as follows:C/N ratio was 14∶1,pH7.63,culture time was 169.69 h,and the yield was 11.67 mg/100 mL.Under this condition,the actual value was 97.04％ of the optimized value,and the results showed that the model was reliable and could be used to optimize the culture condition of Streptomyces sp.YLA0.%以链霉菌YLA0作为出发菌株来优化其高产蓝色素的培养条件,单因素实验的基础上,筛选出C/N比、pH、培养时间三个单因素,利用Box-Behnken的响应曲面设计进一步优化.结果表明,链霉素YLA0高产蓝色素的最佳培养条件为:C/N比为14∶l、pH7.63、培养时间169.69 h,得率11.67 mg/100 mL.该条件下的实际值为优化值的97.04％,说明该模型可靠,可用于链霉菌YLA0高产蓝色素培养条件的优化.