蛋白核小球藻不同生长阶段油脂和脂肪酸组成的分析

摘要

目的:本文通过观察蛋白核小球藻油脂含量及其脂肪酸组成随培养期变化的规律,增加对小球藻油脂代谢机理的了解.方法:收集不同生长时期的蛋白核小球藻藻细胞,采用Folch法和气相色谱法对其在不同生长阶段的总脂含量和脂肪酸组成进行测定.结果:随培养期的延长,油脂含量呈现逐渐上升的趋势,其中缺氮胁迫阶段油脂总含量最高达到细胞干重的21.18％,是对数生长期的1.54倍,稳定期的1.32倍;气相色谱分析结果表明,各个阶段中不饱和脂肪酸的含量均高于饱和脂肪酸,其中缺氮胁迫阶段多不饱和脂肪酸含量最高,占总脂含量的35.90％,其中以油酸C18∶1和亚油酸C18∶2含量较高.结论:缺氮胁迫处理有利于蛋白核小球藻总脂含量的增加和多不饱和脂肪酸的积累,提高了蛋白核小球藻的油脂质量.%Objective:In order to increase more deeply understanding the lipid synthesis of C.pyrenoidosa,the changes of the lipid content and the fatty acid composition with the cultivation stage were observed.Methods:The algal biomass was collected at each cultivation stage by centrifugation of cultivation broth,the lipid was extracted and the fatty acid compositions were analysed.Results:The results showed that the total content of lipid was 21.18％ in the nitrogen deficiency stage,which was 1.54 and 1.32 fold of that at logarithmic growth phase and stationary phase,respectively.The GC results indicated that long chain fatty acids of C 16 ～ C1 8 were the dominant fatty acids of C.pyrenoidosa,and most of them were oleic acid (C18∶1) and linoleic acid(C18∶ 2),accounting for more than 35.90％ of the total fatty acids.Conclusion:Increasing total lipid content and polyunsaturated fatty acid accumulated in nitrogen deficiency stress stage,improving the lipid quality of C.pyrenoidosa.