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杜氏盐藻挥发性物质的HS-SPME-GC-MS分析

     

摘要

The volatile components in Dunaliella Salina were determined by headspace solid phase microextraction(SPME) and gas chromatography-mass spectrum(GC-MS).The result showed that 47 volatile compounds in Dunaliela Salina were isolated and identified.Alcohols,ketones,aldehydes and carboxylic acid were the primary contents,representing 93.69% of the total volatile compounds as described.However,the fragrance components were mainly composed of the productions of3-carotene metabolism or degradation,such as β-cyclocitral,β-ionone,α-ionone and 3,5,5-Trimethyl-3-cyclohexene-1-one etc.Furthermore,the fishy odor compounds primarily included 2-camphanol,trans-bicyclol [4.4.0] decan-1-ol-3-one,4-[2,2,6 -trimethyl-7-oxa-bicyclo [4.1.0] hept-1-yl]-3-butene-2-one,isophorone etc.The pungent odor compounds primarily included furan-3-carboxylcacid-5-tert-butyl-2-p-tert-butyl-phenoxy methyl,5-isopropyl-2-methyl phenol as well as 2,6-diethyl-pyrazine etc.Overall,the fragrance,fishy and pungent odor were the main flavor in Dunaliella Salina.%采用顶空固相微萃取和气相色谱-质谱联用技术,对杜氏盐藻的挥发性成分进行检测结果表明,在检出的47种化合物中醇类、酮类、醛类、羧酸类总合量高达93.69%.其中对杜氏盐藻香味有主要贡献的是β-胡萝卜素的代谢或降解产物,如β-环柠檬醛、β-紫罗兰酮、α-紫罗兰酮、3,5,5-三甲基-3-环己烯-1-酮等.对杜氏盐藻腥味有主要贡献的是2-茨醇、反式-二环[4.4.0]癸烷-1-醇-3-酮、4-[2,2,6-三甲基-7-氧杂二环[4.1.0]庚-1-基]-3-丁烯-2-酮、异佛尔酮等.对杜氏盐藻刺激性气味有主要贡献的是2-对叔丁苯氧甲基-5-叔丁基-3-呋喃甲酸、5-异丙基-2-甲基苯酚及2,6-二乙基-吡嗪等.以上结果表明香味、腥味和刺激味是杜氏盐藻的主要挥发性气味.

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