耙齿菌EA-LJS-73产七叶皂苷培养基配方的响应面法优化

摘要

单因素实验的基础上应用响应面法对产七叶皂苷菌株耙齿菌EA-LJS-73培养基进行方法设计、优化并验证培养基方案,以提高其产七叶皂苷的能力.得到优化后的培养基(1L)如下:淀粉22.660 g,酵母浸粉4.230 g,MgSO47.600 g时,此条件下七叶皂苷浓度的理论值可达到1.116 g/L,验证实验的平均浓度为1.105 g/L,比优化前提高了35％.通过发酵动力学分析得出发酵10 d时,菌株生物量和七叶皂苷浓度达到最高,分别为11.376 g(菌体干重)和1.665 g/L.因此优化的培养基配方合理、可行.%Based on the single factor experiments,response surface methodology was employed to design,optimize and verify the culture medium for a endophytic fungus producing escin which was numbered as Irpex sp.EA-LJS-73,so as to improve the yield of escin.The results of optimum medium were as follows:starch 22.660 g,yeast extract 4.230 g,and MgSO4 7.600 g.Under these conditions,the predicted values of escin concentration could reach 1.116 g/L,the average concentration of the verification test was 1.105 g/L,which was 35％ higher than before.Through the analysis of fermentation kinetics,when the strain fermented 10 d,both the biomass and the concentration of escin were the highest,which were 11.376 g(dry weight of the mycelium)and 1.665 g/L,respectively.Therefore,the optimized medium was reasonable and feasible.